本課程係提供大學部高年級學生及研究生熟悉分子生物應用技術中，重組DNA所應用之原理、方法、儀器及藥品，授課大綱如下：
1. 大腸桿菌固體與液體培養基之配製
Preparation of media for bacteria
2. 大腸桿菌之接種及固體與液體培養
Inoculation and culture of bacteria in solid and liquid media
3.噬菌體固體與液體培養基之配製
Preparation of media for bacteriophage
4. 噬菌體之灑佈培養
Plating of bacteriophage
6. 噬菌體DNA之抽取
Extraction of bacteriophage DNA
7. 噬質體DNA去蛋白處理及定量
Phenol extraction and quantitation of bacteriophage DNA
8. 噬質體DNA之切
Restriction digestion of bacteriophage DNA
9. 製備性瓊脂洋菜凝膠電泳分析及膠體內DNA片段之回收
Electrophoresis and recovery of digested bacteriophage DNA from agarose gel
10. 回收DNA之估量及連結反應
Quantitation of recovered DNA and ligation
11. 待轉型細胞之製備
Preparation of competent cells
12. 轉型質體至大腸桿菌
Bacterial transformation
13. 轉型效率之估算，藍白篩選之觀察及接種
Determination of efficiency of transformation, observation of blue-white colony selection and inoculation
14. 質體DNA之小量製備
Minipreparation of plasmid DNA